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Effects of Salinomycin on Proliferative， Invasive and Metastatic Abilities of Hepatoma Cell Line HepG2 in vitro
Wang Jingyu1,3, Wang Yichen2, Wang Li3, Wang Zhiping1,2*
1School of Life Sciences, Lanzhou University, Lanzhou 730000, China; 2Institute of Urology, Second Hospital, Lanzhou University, Lanzhou 730030, China; 3Institute of Pathophysiology, School of Basic Medical Sciences, Lanzh
Abstract: To investigate effects of salinomycin on the proliferative， invasive and metastatic abilities of hepatoma cell line HepG2 in vitro and analyze the possible mechanisms， MTT was used for determining the proliferative abilities of HepG2 and human normal liver L02 cells. The cytoskeleton was observed using confocal laser scanning microscopy after F-actin staining by FITC-labeled phalloidin. The invasive and migratory abilities were detected by Transwell assay. The protein expressions of β-catenin， MMP-2 and MMP-9 at protein levels were evaluated by Western blot. The results showed that the treatment of L02 cells with salinomycin (0~8 μmol/L) for 24 h and 48 h had no significant effects on cell viability. However， treatment of similar doses of salinomycin suppressed the growth of HepG2 cells in a time and concentration dependent manner. HepG2 was treated by salinomycin (1， 4 μmol/L) for 24 h before invasive and migratory abilities were examined. Compared with that in the control group， the number of invasive and migratory cells was remarkably decreased (P<0.05)， the structure of F-actin microfilament cytoskeleton was significantly changed. In addition， immuno-blot showed that the expressions of β-catenin， MMP-2 and MMP-9 at protein levels were significantly down-regulated (P<0.05). The results suggested that the proliferative， invasive and migratory abilities of hepatoma cells could be inhibited by salinomycin， which may be related to down-regulating the protein expressions of β-catenin， MMP-2 and MMP-9.
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