The Exploration of the Law about the Proinflammatory concentration in the Serum of Mastitis Model Rats after Injecting Bone Marrow Mesenchymal Stem Cells and Antibiotics

Abstract: The aim of the study is to invistigate the effects on serum mainly proinflammatory levels of experimental mastitis that transplanted rat bone marrow mesenchymal stem cells (BMSCs) through breast base，mainly proinflammatory contains tumor necrosis factor-α (TNF-α)， interleukin-1β (IL-1β)， interleukin-2 (IL-2)，interleukin-6 (IL-6)， interleukin-8 (IL-8). 5 rat selected randomly from 70 normal female SD rats were killed after 72 h of postpartum namely test –1 d， the rest of 65 rats were perfused 0.05 mL (50 μg) endotoxin through the nipple tube as the reference to domestic common way， 5 rats that the data obtained from these rats would be equally compared and contrasted with data from other groups later were randomly selected and killed after 1 d namely test 0 d that established experimental mastitis model successfully， at the same time the rest of the 60 rats were randomly divided into three groups: control group (injection of saline in breast base， 50 μL)， antibiotic group [injection of antibiotic in breast base， 50 μL， (5 000 U/side)] and BMSCs transplantion group [injection of BMSCs in breast base， 50 μL (1×106)]， n=20， and killed 5 rats each group at 1， 3， 5， 7 d， respectively. The mammary gland tissues were collected immediately， fixed for tissue section. Cardiac blood was collected from the dead bodies and preserved for detecting TNF-α， IL-1β， IL-2， IL-6， IL-8. The results showed that tranplanted BMSCs can significantly lower the levels of TNF-α， IL-1β， IL-8， promote the repairion of mammary gland tissue， and this provided a new way for mastitis prevention.

CSTR: 32200.14.cjcb.2013.05.0013