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Cell Growth Inhibition by Exogenous miR-34a in H1299 Cell
Duan Guangxin1, Duan Weiming2, Xu Yaxiang1, Zhang Yanjuan3, Zhou Xinwen3*
1School of Biology and Basic Medical Science, Medical College of Soochow University, Suzhou 215123, China; 2The First Hospital Affiliated to Soochow University, Suzhou 215123, China; 3School of Radiation Medicine and Prote
Abstract: To elucidate the function and mechanism of exogenous miR-34a involved in growth， apoptosis and senescence in the p53–/– H1299， miR-34a mimics was used to transfect into H1299. Viablity cell rate was analyzed by MTT. Erythrosine B cells staining was used to count the rate of cell death. Apoptosis was detected by Annexin V/PI staining. Senescence was observed by senescence associated β-galactosidase kit staining. The expression of the Bcl-2， Puma， E2F3 and Cdk4 was resolved by Western blot. The group transfection with miR-34a has a lower cell viablity rate than the group of negative control. The decrease of cell viablity of 48 h is more notable than 24 h. The cell viablity rate of 24 h and 48 h are 79.94%， 64.83%， respectively. The rate of cell death and apoptosis is higher and the senescence staining more marked when transfected with miR-34a. The expression of Bcl-2， E2F3 and Cdk4 significantly decreases and Puma increases when induction of miR-34a. So miR-34a can decrease it’s target proteins of p53 downstream and induce apoptosis and cell death， senescence， inhibit cell growth of H1299 cell by partly saving the p53 pathway and p53 independent pathway.
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