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Enhancement of the Growth Inhibiting Effect to Liver Tumor Cell Hep3B by Combining Parthenolide with ZD55-TRAIL
Li Xiaoyan1, Ma Hui2, Pan Qiang1, Yang Xinyan1, Wu Liqin1, Chen Kan1*
1Xinyuan Institute of Medicine and Biotechnology, College of Life Science, College of Life Sciences Zhejiang Sci-Tech University,Hangzhou 310018, China; 2Department of Medicine, Hanzhong Vocational and Technical College,Shaanxi Provi
Abstract: This study aimed at studying the anti-tumor effect in hepatocarcinoma cell Hep3B cells by combining parthenolide (PTL) with ZD55-TRAIL. The experiment was constructed oncolytic adenovirus ZD55-TRAIL by Adeasy systerm. Then MTT， crystal violet and Hoechst33342 staining assay were used to assess the growth inhibition effects of single or combination therapy on liver tumor cell Hep3B and human normal cell QSG-7701. The results showed that 10 MOI ZD55-TRAIL combining with PTL treated for 72 h， Hep3B cells’ survival rate is only 44%， and no significant damage to the QSG-7701 cells， while in ZD55-TRAIL and PTL 10 MOI separately treated cells， the survival rate is 70% and 66%， respectively. The crystal violet and Hoechst33342 staining results showed that the combined treatment group Hep3B apoptotic features more obviously. Oncolytic adenovirus ZD55-TRAIL joint PTL had significant destruction in vitro on hepatoma cells Hep3B， but no significant side effects to QSG-7701 cells.
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