Home > Browse Issues > Vol.35 No.5
Studies of Drosophila Golgin-84 Expression and Functions in Development
Hou Guoli1, Lin Xinhua2, Wu Yihui2*, Diao Aipo1*
1School of Bioengineering, Tianjin University of Science and Technology, Tianjin 300457, China; 2State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
Abstract: The structure of Golgi apparatus is closely related to membrane transport and normal cellular function. It is already known that the cis-Golgi localized Golgin-84 plays an essential role in the maintenance of Golgi structure in mammalian cells. However， the function of Golgin-84 in development remains unclear. To examine the roles of Golgin-84 in development， we generated and purified the polyclonal antibody against Drosophila Golgin-84，and the UAS-golgin-84-V5 over-expression and golgin-84 RNAi transgenic Drosophila lines were generated. We preliminarily analyzed the function of golgin-84 in Drosophila melanogaster during wing development. Western blot and immunofluorescence staining showed that the purified antibody can recognize recombinant and endogenous Golgin-84 protein， and the antibody stained Golgin-84 co-localized with Golgi marker protein GM130 in S2 cells. Golgin-84 was ubiquitously expressed during development in Drosophila melanogaster and abundant in testis. Furthermore， inhibition of Golgin-84 expression resulted in wing defects， and wing growth and patterning defects were observed in the Golgin-84 over-expressed Drosophila. In conclusion， we successfully generated the Golgin-84 antibody and transgenic lines in Drosophila， and examined the function of Golgin-84 in wing development. Our results laid the foundation to further study the physiological function and molecular mechanism of golgin-84.
CN
EN