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Effects of Quercetin on the Proliferation and Cell Cycle in Fagopyrum tataricum to Human Gastric Carcinoma Cell Line SGC-7901
Li Yuying1, Zhao Shujuan1, Bai Chongzhi2, Cui Xiaodong1, Wang Zhuanhua1*
1Key Laboratory for Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University,Taiyuan 030006, China; 2Central Laboratory of Shanxi Academy of Traditional Chinese Medicine, Taiy
Abstract: Quercetin was extracted from F. tataricum for investigating its effect on cell proliferation， apoptosis， migration and cell cycle in the human gastric carcinoma cell line SGC-7901. The possible effects of quercetin on the proliferation of 293T， SGC-7901， HepG2 and HeLa cell lines were investigated by MTT assays. Effects of quercetin on cell migration were investigated by cell injury healing experiment. Effects of quercetin on cell cycle and cell apoptosis were investigated by flow cytometry. The cell cycle regulation factors were investigated by RTPCR. As a result， MTT assay showed that quercetin could specifically inhibit the viability of 293T， SGC-7901， HepG2 and HeLa cells， while it had showed less toxicity against 293T cells and more toxicity against SGC-7901 cells. Cell injury healing experiment indicated that quercetin could suppress cells migration. The results of flow cytometry showed that the cells of G1 phase and S phase decreased， the cells of G2/M phase increased and the ratio of apoptosis cells increased. The results of RT-PCR showed that the transcription level of p53 increased and CyclinD1， CDK2， CDK7 decreased. Together all， quercetin in F. tataricum can induce apoptosis， arrest cell cycle and inhibit cell proliferation and migration of SGC-7901 cells.
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