Influence of rpoE Gene on Growth Ability of Salmonella enteritidis under the Stress of Circumstance

Abstract: rpoE gene is one kind of regulatory genes which can be commonly found in bacteria and plays an important role to various environmental stress response. In this study， an rpoE gene mutant of Salmonella enteritidis (S.E) called ΔrpoE was constructed by λ-Red recombinase system and the growth ability of ΔrpoE under different stress of circumstance was inspected. The results showed that there was no difference between wild and mutant strains at 37 °C. However， the growth ability of mutant strains was significantly weaker than wild strains under the culture condition of high and low temperature， acidic， oxidative， high-osmotic. In summary，this is the first time to get the rpoE knock-out mutant in Salmonella enteritidis and the function of rpoE gene is preliminarily inspected， which can help to find out the further function and mechanism of the rpoE gene in Enterobacteriaceae such as E.coli and S.E.

CSTR: 32200.14.cjcb.2013.05.0004