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The Effect of Starvation on Migration and Pseudopod of Dictyostelium discoideum
Guo Xiaohui1, Zhao Min2,1, Wang Xiaoyan1, Zhao Sanjun1, Gao Runchi1,3, Gao Jing1, Shi Limin1*
1Regeneration Biology Research Section,Yunnan Normal University, Kunming 650500, China;
2Department of Dermatology and Department of Ophthalmology, Institute for Regenerative Cures, School of Medicine, niversity of California at D
2Department of Dermatology and Department of Ophthalmology, Institute for Regenerative Cures, School of Medicine, niversity of California at D
Abstract: We studied the character of movement and pseudopodia of Dictyostelium discoideum to investigate the relationship between migration and pesudopod of Ddiscoideum under starvation. The results showed that the movement speed and direction persistent of cell increased significantly with the starved time. The cells moved with maximum speed (8.36 μm/min) and strongest persistent when they were starved for 3 h and 5 h， respectively.The average formation speed of pseudopod also increased significantly with the starved times and reached maximum speed (5.18 μm/min) when cells were starved after 3 h. The splitting pseudopodia were produced at maximum ratio at 73.55% by the cells that were starved for 5 h. The results of Pearson correlations indicated that the migration speed of cells related positively with formation speed of pseudopodia. The persistence of cell migration correlated positively with the ratio of splitting pseudopod. The cell migration was not influenced significantly by extension time，extension area and extension size of pseudopod.
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