Home > Browse Issues > Vol.35 No.4
A Study on the Effect and Mechanism of O-GlcNAc Transferase Mediated O-Glycosylation in Ovarian Cancer Cell Migration
Jin Fengzhen1, Yu Chao2, Yang Zhu1*
1The Second Afiliated Hospital of Chongqing Medical University, Chongqing 400010, China;
2Institute of Life Science, Chongqing Medical University, Chongqing 400016, China
2Institute of Life Science, Chongqing Medical University, Chongqing 400016, China
Abstract: In present study， we explore the effect and molecular mechanism of O-GlcNAc transferase (OGT) mediated O-Glycosylation in the ovarian cancer cell migration. We established low-expressed O-Glycosylation ovarian cancer HO-8910PM cell models induced with OGT gene interference and high-expressed O-Glycosylation ovarian cancer OVCAR3 cell models induced with OGA inhibitors; the expression of matrix metalloproteinase-2 (MMP-2)， MMP-9， OGT and total O-Glycosylation level were detected by qPCR and Western blot assays; the migration of ovarian cancer cells were observed using transwell cell migration assays in vitro. Our results showed that the migration ability of OVCAR3 cells was obviously enhanced by OGA inhibition and the migration potential of HO-8910PM cells was dramatically reduced by OGT interference; the mRNA levels of MMP-2 and MMP-9 in HO-8910PM cells were significantly reduced by OGT interference. In summary， we describe a novel role for the O-Glycosylation mediated by OGT in the migration of human ovarian cancer cells through， in part， increasing the expression of MMP-2 and MMP-9 mRNA levels.
CN
EN