Study of Damnacanthal on Anti-proliferation， Cell Migration Inhibition and Its Mechanism in Human Hepatoma Cell HepG2

Abstract: The trypan blue staining， wound healing assay， specific fluorescence staining are used to investigate the effect of proliferation， cell migration， microfilaments cytoskeleton， keratin filaments network and apoptosis induced by damnacanthal on human hepatoma cells HepG2 respectively. The results demonstrated that damnacantha1 significantly inhibited cell proliferation of human hepatoma cell HepG2 at concentrations of 15~60 μmol/L in time and dose dependent manners; 15 μmol/L damnacanthal inhibits cell migration and 30 μmol/L almost inhibits completely; after HepG2 cells were exposed to damnacanthal (25， 27.5， 30， 35 μmol/L) for 24 h， stress fibers， lamellipodia and filopodia were absent or decreased gradually with increased concentrations， which is similar to the results caused by 8μmol/L cytochalasin B in the same condition; however， 25 μmol/L and 30 μmol/L damnacanthal had only slightly influence on keratin filaments of HepG2; 15~60 μmol/L damnacanthal induced apoptosis of HepG2 in differently degrees. The destructive effects of damnacanthal on HepG2 cells stress fiber (microfilaments) may be the direct reason of anti-proliferation and cell migration inhibition， maybe the direct reason of cell adherence decreased or lost and further lead to HepG2 cells apoptosis as well.

CSTR: 32200.14.cjcb.2013.04.0008