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Applications of Single Cell Technologies in Stem Cell Research
Dong Fang, Yuan Weiping, Cheng Tao*
State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China
Abstract: Stem cells are a heterogeneous cell population characterized by their ability of self-renewal and
differentiation. Stem cell research at the cell population level can not meet the demand for further understanding the
biology and its applications. Recently， the single cell-based technologies are rapidly applied and advanced in stem
cell and related studies. This review highlights the current technologies on single cell separation， single cell clonal
assay， single cell transplantation， single cell real-time PCR and single cell sequencing with an emphasis on their applications，
as exemplified in hematopoietic stem cell research.
differentiation. Stem cell research at the cell population level can not meet the demand for further understanding the
biology and its applications. Recently， the single cell-based technologies are rapidly applied and advanced in stem
cell and related studies. This review highlights the current technologies on single cell separation， single cell clonal
assay， single cell transplantation， single cell real-time PCR and single cell sequencing with an emphasis on their applications，
as exemplified in hematopoietic stem cell research.
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