A Study on the in vitro Anti-cancer Effects by Targeting OCT4 Pluripotent Gene in Lung Adenocarcinoma Stem Cells

Ning Renli¹, Huang Jinsu², Wu Lixia¹, Li Rong², Xu Huili¹, Zhou Jin¹, Dong Qianggang^1*

¹Laboratory of Cancer Stem Cells, Shanghai Cancer Institute, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200032, China; ²Department of Pulmonary Medicine, Shanghai Chest Hospital, Shanghai Jiaotong Unive

Abstract: Octamer-binding transcription factor 4 (OCT4) is a pluripotent gene critical for the maintenance of self-renewal in stem cells. In recent years， emerging evidence in clinic has indicated that the presence of OCT4 in lung adenocarcinoma portends a dismal prognosis for patients， suggesting OCT4 as a potential target for the treatment of this tumor. With microRNA-mediated gene silencing and cDNA microarray techniques， we reported herein that OCT4 is functional for transcriptional regulation in lung adenocarcinoma cells. The studies showed that treatment with 15-deoxy-delta12，14-Prostaglandin J2 (15d-PGJ2) and desferrioxamine (DFO) in combination significantly down-regulated the expression of OCT4 and inhibited the proliferation and colony formation in lung adenocarcinoma cells. These data documented that pharmacological regulation of OCT4 expression possesses a markedly anti-cancer effects， at least in the in vitro setting. Thus， the results provide a novel avenue for exploring targeted therapies in lung cancer.

CSTR: 32200.14.cjcb.2012.11.0005