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Establish An in vitro HCT116 Human Colon Cancer Cell Invasion Model by TNF-α
You Zhenyuan, Chen Danyang, Liu Chuqi, Yuan Huimin, Wang Hongsheng, Du Jun*
Department of Microbial and Biochemical Pharmacy, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China
Abstract: This research focused on establishing an in vitro HCT116 cells invasion model for drug screening. In this study， HCT116 human colon cancer cells were treated by 20 ng/mL TNF-α for 7 days， then the expression of CCR7 and CXCR4 receptors were detected by flow cytometry. The cell invasion ability of CCL21 and SDF- 1 was further evaluated by transwell chamber. After stimulated by 20 ng/mL TNF-α for 7 days， the expression of CCR7 and CXCR4 increased significantly which facilitated the migration of HCT116. Furthermore， the CCL21 and SDF-1 could enhance the cell invasiveness. The results of this study demonstrate that the invasiveness of HCT116 can be enhanced by TNF-α， so that it can be used to establish an invasion model in vitro for drug screening.
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