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Distribution of ABA and AhNCED1 in Peanut Leaves of Different Drought Resistant Cultivars Subjected to Drought Stress
Hu Bo, Xiao Suni, Lü Yan, Li Ling*
Guangdong Provincial Key Lab of Biotechnology for Plant Development, College of Life Sciences, South China Normal University, Guangzhou 510631, China
Abstract: In this study, the changes of ABA distribution and ABA content in leaf of two species of the peanut (Yueyou 7 and Shanyou 523) were researched through the immunoenzyme localization and ELISA methods. The results showed that the ABA distribution and ABA content in peanut leaf were increased after drought stress. The synthesis of ABA and AhNCED1 in the leaves of Yueyou 7 appeared more quickly than that in the susceptible cultivar (Shanyou 523). Furthermore, AhNCED1 transcript and protein in Yueyou 7 were induced more than that in Shanyou 523, coinciding with higher ABA accumulation. After treated with Naproxen, ABA distribution were lower than that treated with PEG. These results showed that the vascular cambium is the main region of leaf in peanut response to drought stress, and the higher drought-resistant of Yueyou 7 peanut may be relate to the more distribution of ABA and AhNCED1 in leaf.