Effects of X-ray on Activity of Digestive Enzyme and Expression of Bax and Ghrelin in Stomach of Fifal Mice

Abstract: To explore the effects of X-ray on activity of pepsin， lipase of duodenum and expression of Bax and Ghrelin in stomach of fifal mice， 170 mice were irradiated with different dosages (0， 4， 12， 20， 28 Gy) of X-ray. At 1~20 d after irradiation， detected activity of pepsin and lipase by colorimetry， studied distribution and positive expression of Bax and Ghrelin by immunohistochemical technology and analyzed them by IPP5.0. Results showed that X-ray radiation affects activities of pepsin， lipase and expression of Bax and Ghrelin in stomach of fifal mice. In irradiated group， the activities of pepsin are higher than control group on the first day after irradiated in stomach， and in other periods the activities are always lower than control group of fifal mice. In irradiated group， the activities of lipase of duodenum are all always lower than control group. Bax protein immunoreaction appeared in epithelial cells of stomach mucosa， the intensity increased with dosages augment. Ghrelin immunoreaction appeared in gland cells of stomach mucosa， the intensity reduced with dosages augment. X-ray radiation affects the activity of digestive enzyme of fifal mice， and this effect might be correlated with the change of positive expression of Bax protein and Ghrelin in stomach.

CSTR: 32200.14.cjcb.2012.09.0002