Home > Browse Issues > Vol.34 No.7
The Effect of RCMV Infecting Astrocytes on Neural Stem Cells Differentiation
Sun Xiuning1, Shi Lihong2, Liang Ruiwen1, Liu Zhijun3*, Li Ruifang1, Guan Yingjun4 , Yu Li4, Guan Zhiyu1
1Department of Parasitology, Weifang Medical University, Weifang 261053, China; 2Department of Pharmacology, Weifang Medical University, Weifang 261053, China; 3Department of Microbiology, Weifang Medical University, Weifa
Abstract: To investigate the effects of rat cytomegalovirus (RCMV) infected astrocytes on neural stem cells (NSCs) differentiation， the astrocytes and embryonic hippocampal neural stem cells were isolated and primary cultured from SD rats. NSCs were co-cultured with RCMV infected astrocytes in Transwell 24-well plate system with respective control groups. After NSCs were co-cultured with RCMV infected astrocytes， immunofluorescence staining was performed to study the expression of MAP2 and GFAP on neurons and astrocytes， respectively. The results showed that after co-culturing astrocytes with NSCs， the NSC differentiation slowed down and the differentiated neurons to astrocytes ratio was lower compared with the control group， suggesting that astrocytes infected with RCMV can inhibit the differentiation of NSCs. The possible mechanism may be related with the nutritional factors secreted from astrocytes， but affected by RCMV infection and thus interfered the differentiation process of NSCs.
CN
EN