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E3 Ubiquitin Ligase Gene CG4911 Gene Knock-out and Function Analysis
Ye Xiaolei1, Fan Xiaolan1, Wang Shuangshuang1, Zhang Yan2, Jin Longjin3, Lin Xinhua2*
1School of Ophthalmology and Optometry, Wenzhou Medical College, Wenzhou 325027, China; 2State Key Laboratory of Biomembrane and Membrane Biotechnology, Key Laboratory of Stem Cell and Developmental Biology, Institute of Zoology, Chi
Abstract: Protein ubiquitination is an important post-translational modification process involved in cell cycle regulation， gene transcription， signal transduction， inflammation and stem cell maintenance. Ubiqutin ligase E3 is an essential enzyme and plays important role in ubiquitin-proteasome process. However， little is known about its function and molecular mechanism of E3 ligase during development. In this study， Drosophila melanogaster was used as a model to study the function and molecular mechanism of gene CG4911， which is one of the important ubiquitin ligases genes. We have generated mutant for CG4911 and construct CG4911-pUAST-V5 vector as well as its transgenic lines. The result showed that CG4911 mutant is viable. The protein of CG4911 localized in the cytoplasm and there was no modification happened with CG4911 when expressed in Hela cells. Furthermore， over expression of CG4911 leads to the notum developmental defects. In summary， this is the first time to get CG4911 knock out mutant and CG4911 transgenic fly. Also， it’s the first time to uncover a novel F-box gene CG4911 gene function in developmental contaxts. Our studies provide basis to further clarify the function of ubiquitin ligases and molecular mechanisms.
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