The Application Example of Autophagy Induction and Testing Technology in Course of Cell Biology Experiment

Abstract: Cell autophagy is a highly conservative intracellular process， which degradates its own composition with lysosome. It relates to many physiological functions. It degradates excess or abnormal macromolecular during starvation， stimulation or something unfit for survival， in order to provide energy and raw materials for cells survival， promote the growth and development of the organism and cell differentiation， and respond to the environment changes. However， excessive autophagy can cause cell death， so autophagy is also known as type II programmed cell death， which is a new way to cause cell death besides apoptosis and necrosis. In addition， abnormal autophagy relates to a variety of pathological process such as cancer， neural degenerative diseases and pathogen infection， etc. Autophagy plays an important role in physiological and pathological process， so it becomes a new hotspot in the research of cell biology. In this paper， with autophagy as the research subject， we design the experiment about autophagy induction and observation suitable for undergraduate students. This paper introduces the experiment principle， the experiment design and arrangement in details， which provides valuable references for the popularization of this technology in experiment course.

CSTR: 32200.14.cjcb.2012.03.0012