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High-frequency Floral Bud Regeneration from Petal Segment Cultures of Sinningia speciosa Hiern
Pang Jiliang1*, Wang Lilin1, Xiang Taihe1, Zhong Dan1, Yu Hong2
1College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China;2Institute of Biotechnology, Hangzhou Academy of Agricultural Science, Hangzhou 310024, China
Abstract: The influence of gibberellin and cytokinin on the regeneration of floral bud from petal segments of Sinningia speciosa Hiern in vitro was studied here. Two types of regeneration were regeneration of floral buds only (designated BF)， and regeneration of both floral and vegetative buds (designated BF+V). The capacity of floral bud regeneration was influenced by light， the size of the floral bud and exogenous gibberellin (GA3) and cytokinin in the media. In MS basic medium containing 1.0 mg/L GA3， the addition of 6-benzyladenine (BA， 0.5 mg/L) significantly increased the frequency of total (BF+BF+V) flower bud formation to 100%. The culture was in darkness， the frequency of BF regeneration was up to 93.4%. The highest percentage of BF was 86.7% formed in petal segments from 7 mm floral buds in diameter. Morphological changes in tissue structure of petal segment cultures were observed at several stages (0 to 30 d) under the light microscopy.
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