Construction and Expression of the Recombinant Inducible Tumor-targeted Adenovirus Containing IFN-α2a

Abstract: Adenovirus-based vector， which is one of the widely used gene transfer tools， especially applied in metastatic tumor gene therapy， shows a very promising prospect. However， these tumor-targeted vectors lack of controllability， and their efficacy and safety are greatly affected. Therefore， the development of new inducible tumor- targeted recombinant vectors has attracted much attention in recent years. Here， we construct the inducible tumor- targeted recombinant adenovirus containing IFN-α2a. The recombinant adenovirus can infect many tumor cell strains such as HepG2 cells efficiently. RT-PCR and Western blot results showed that the IFN-α2a gene expressed specifically in tumor cells， while hardly expressed in non-tumor cells such as L02. After induction with ZnSO4， the expression levels of IFN-α2a are higher than those no inducted. The inducible tumor-targeted recombinant adenovirus containing IFN-α2a can be further studied for inhibition effect on cancer in vitro and in vivo.

CSTR: 32200.14.cjcb.2012.03.0009