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NMDA Receptor Activation Regulates Wnt/β-catenin Signaling Pathway in Primary Cortical Neurons
Peng Yanxi1, Wan Xianzi1, Li Yichen1, Li Shasha1, Zhang Wei2, Tang Shaojun3, Zhong Ling1*
1School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China;2Center of Experiment Animal, Sun Yat-sen University, Guangzhou 510080, China; 3Department of Neuroscience and Cell Biology,University of
Abstract: Wnt signaling has a key role in regulation of synaptic formation and function in the brain. Glutamate， which is the mainly excitatory transmitter， binds the glutamic acid receptor to regulate the activity of many signaling pathways. To determine the effect of NMDA receptor activation on Wnt/β-catenin signaling， primary cortical C57 mouse neurons were treated with 10 μmol/L MSG and 50 μmol/L NMDA and the components of Wnt/ β-catenin signaling were analyzed by Western blot or immunofluorescent experiments. We found that NMDA receptor activation not only increased the p-Ser9-GSK-3β to inhibit its activity but also caused an increase of intracellular β-catenin and induced its translocation into nuclei， thereby up-regulated the downstream gene expression. These results suggest that NMDA receptors activation up-regulates Wnt/β-catenin signaling in primary cortical cultured neurons.
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