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Cloning and Expression Patterns of PtSUS1 in Populus tomentosa
Li Ying, Chen Zhong, Li Hao, Guo Bin, Wang Jia, An Xinmin*
National Engineering Laboratory for Tree Breeding, NDRC; Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, MOE; Tree and Ornamental Plant Breeding and Biotechnology Laboratory of State Forestry Adminis
Abstract: Sucrose synthase (SUS) was closely associated with sink strength， secondary wall formation and cellulose synthesis in plant. Among these items， cellulose synthesis is considered to be the most important. According to the known gene fragment of PtSUS1 and the principle of RACE amplification， we got the gene sequence of PtSUS1 from Populous tomentosa. The full sequence of PtSUS1 which contains an open reading frame (ORF) is 2 669 bp encoding a deduced amino acid sequence of 805 residues. Blast analysis results indicated that the homology was as high as 76% to 97% and 81% to 97%， respectively， when compared PtSUS1 sequence of amino acid and nucleotide with the ones of Arabidopsis thaliana， Eucalyptus grandis， Gossypium hirsutum， Citrus unshiu， and Populus trichocarpa. Furthermore， secondary structure and functional sites in amino acid sequences have been predicted by the bioinformatics technology， the results suggested that the encoded protein contains 2 functional domains， 38 PKA phosphorylation sites and no transmembrane regions. Phylogenetic analysis demonstrated that PtSUS1 is most similar to PtSUS2. And the expression of PtSUS1 mRNA was detected in all the tissues and organs tested such as root， stem， leaves and flowers of Populus tomentosa by RT-PCR， presenting the constitutive expression pattern. The data of this paper will provide the basis for further gene function research of PtSUS1 in Populus tomentosa.
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