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Expression of mTOR in Different Developmental Stages of SD Rat， and the Research of Relationship between mTOR and Spermatogenesis
Xu Hao, Chen Xuemei, Ding Yubin, He Junlin, Wang Yingxiong, Yang Dehui, Liu Xueqing*
Reproductive Biology Laboratory, School of Public Health, Chongqing Medical University, Chongqing 400016, China
Abstract: Spermatogenesis was very important in male reproductive and the proliferate of spermatogonia ensure the spermatogenesis successfully. As the growth of the age， the quantity and quality of sperm reduce gradually. mTOR signaling pathway played a central regulatory role in cell proliferation and differentiation. Therefore， mTOR signaling pathway played an important role in spermatogenesis probably. To investigate the relationship between the mTOR signaling pathway and spermatogenesis， we did the SD rat testis tissue sections by immunohistochemistry and we found mTOR expressed in the spermatogonia. Then， we detected the expression of mTOR mRNA in the different developmental stages of rat testis by FQ-PCR， and the results showed the significant differences of transcription between 80 weeks group and 8 weeks group. At last， we found the expression of mTOR and the phosphorylation efficiency of P70S6K reduced gradually as the growth of the age by Western blot. After treated with rapamycin， the P-P70S6K/P70S6K and the number of sperm reduced in the normal 8 weeks SD rat. These results convinced us that mTOR signaling pathway was very important in the spermatogenesis.
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