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Influence of SOCS-1 on IL-1β-induced Renal Tubular Epithelial- Myofibroblast Transdifferentiation
Liu Qingjuan1, Xing Lingling2, Li Jianying3, Hao Jun1, Liu Wei1, Wang Pin4, Liu Shuxia1*
1Department of Pathology, Hebei Medical University, Shijiazhuang 050017, China; 2Department of Nephrology, Second Hospital, Hebei Mdical University, Shijiazhuang 050000, China; 3Department of dermatology, People’s Hospital
Abstract: In order to investigate the effect of suppressor of cytokine signaling-1 on renal epithelialmyofibroblast transdifferentiation induced by interleukin-1β in human renal tubular epithelial cells， we constructed stable transfection of HKC with pCR3.1/SOCS-1. The cells were collected after stimulated 48 h with IL-1β (10 ng/mL). The protein and mRNA were observed by immunocytochemistry， Western blot， enzyme-linked immunoadsorbent assay (ELISA) and reverse transcription and polymerase chain reaction (RT-PCR). The results suggested that after stimulated by IL-1β， the expression levels of α-SMA， p-STAT1， ColI and FN were significantly increased. however， the expression of CK18 decreased in control group and transfected with pCR3.1 vector group. Overexpression of SOCS-1 inhibited IL-1β-induced high expression of α-SMA， STAT1， ColI and FN. Meanwhile， overexpression of SOCS-1 reversed the expression of CK18 in HKC with IL-1β stimulation. We conclude that overexpression of SOCS-1 inhibits IL-1β-induced renal tubular epithelial-myofibroblast transdifferentiation maybe partly through blocking activation of STAT1.
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