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Effects of High Glucose and LY294002 on the Expression of Collagen IV in Mouse Podocyte
Xing Lingling1,2, Liu Qingjuan1, Fu Shuxia2, Cao Yanping3, Liu Wei1, Duan Huijun1*
1Department of Pathology, Hebei Medical University, Shijiazhuang 050017, China; 2Department of Nephrology,the Second Affiliated Hospital of Hebei Medical University, Shijiazhuang 050000, China;3Department of Nephrology, th
Abstract: To investigate effects of high glucose and phosphoinositide 3 kinese/protein kinase B (PI3K/ Akt) pathway on the expression of collagen IV (Col IV) in mouse podocyte， we divided cultured mouse podocytes into high glucose (30 mmol/L， HG) group and normal glucose (5 mmol/L， NG) group. Cells were collected respectively at 0， 12， 24， 48 h after stimulation. The expression of collogen IV was detected by immunocytochemistry and Western blot analysis. The expression of phospho-Akt and the inhibition of LY294002 on collogen IV were analized by Western blot. Compared with control group， the level of Col IV in mouse podocytes treated by high glucose was significantly increased (P<0.05)， with reaching the peak at 24 h. The phosphorylation of Akt was observed in mouse podocytes induced by high glucose. The level of phospho-Akt was increased in a time-dependent manner. However， inhibition of activation of Akt with LY294002， a specific PI3K/Akt pathway inhibitor， attenuated the high glucoseinduced expression of Col IV at 24 h after intervention. We conclude that high glucose maybe up-regulate the expression of Col IV in mouse podocytes by activating PI3K/Akt pathway.
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