Home > Browse Issues > Vol.33 No.11
Induction and Identification of Mesenchymal Precursor Cells from Cynomolgus Monkey Embryonic Stem Cells
Ren Zhenhua1,2, Wang Jiayin1, Zhu Wanwan1, Zou Chunlin1, Zhang Y. Alex1*
1Cell Biology Laboratory, Xuanwu Hospital, Capital Medical University, Beijing 100053, China; 2Department of Anatomy, Anhui Medical University, Hefei 230032, China
Abstract: Mesenchymal stem cells (MSCs) have received considerable attention in recent years for various therapeutic approaches. The reason is that MSCs have the ability to expand in vitro and differentiate into osteogenic， adipogenic and chondrogenic lineages， which could then be used for cell therapy and tissue engineering. Embryonic stem cells (ESCs) are pluripotent cells with unlimited proliferation and differentiating into any cell type of the body. In this study， cynomolgus monkey ESCs were cultured in the absence of serum to form embryoid bodies (EBs)， and then EBs were transferred for adherent culture in the presence of serum. Mesenchymal precursor cells (MPCs) migrated from EBs were selectively isolated and expanded. The results showed that MPCs derived from ESCs had the biological characteristics of mesenchymal stem cells (MSCs)， and could be differentiated into osteogenic and adipogenic cells. NOD/SCID mice injected with MPCs did not show signs of tumor development. The results suggested that MPCs derived from cynomolgus monkey ESCs had certain the safety of its clinical application.
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