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Induction of Dental Pulp Stem Cell Odontogenic Differentiation by Overexpression of Msx1， Pax9 and Bmp4
Huang Yide1,2#*, Wang Fangfang1#, Hu Xuefeng1,2
1College of Life Science, Fujian Normal University, Fuzhou 350108, China; 2College of Life Science, Fujian Normal University,Fujian Key Laboratory of Developmental and Neuro Biology, Fuzhou 350108, China
Abstract: Tooth regeneration based on stem cells is a promising biological strategy in tissue engineering. Studies showed that odontogenesis involves in epithelial-mesenchymal interaction in developing embryo， tooth regeneration using stem cells also requires both epithelium-derived stem cells and mesenchyme-derived stem cells. In recent studies， dental pulp stem cell (DPSC)， which were isolated from dental pulp tissues， showed excellent pluripotency potential， and might be used as suitable mesenchyme-derived stem cells for tooth regeneration. In order to induce DPSCs into odontogenic lineage， we over-expressed Msx1， Pax9 and Bmp4 by lentivirus-mediated method in DPSCs， respectively. Our results showed that over-expression of Msx1， Pax9 and Bmp4 induced expression of dentin sialoprotein， osteocalcin and osteopontin， differentiation markers for odontoblasts， formed mineralized tissues， and all leaded to an elevated alkaline phosphatase in DPSCs in vitro. The ability to induce differentiation of DPSCs into odontoblasts has some difference for Msx1， Pax9 and Bmp4. Over-expression of Msx1 had the most effective induction on odontogenic differentiation in DPSCs in vitro， the second was over-expression of Bmp4， Pax9- expressing DPSCs didn’t obviously express osteopontin and form calcified tissues.
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