Home > Browse Issues > Vol.33 No.8
Establishment of Genetic Transformation System for Cucumber with Mn-SOD Gene
Fan Aili1,2, Sun Yan1*, Guan Qingmei1, Liang Dong1
1College of Horticulture, Northwest A&F University, Yangling 712100, China; 2Vegetable Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China
Abstract: In this study， several factors affecting transformation rate were studied by Agrobacteriummediated in Nong cheng No.3 cucumber. These factors included sensitivity of cotyledonary nodes to antibiotics (Kan and Cef)， days of pre-culture， concentrations of Agrobacterium tumefaciens， days of co-culture and time of being infected on the optimum culture medium and so on. The high frequency transgenic regeneration system of cucumber was established as follows: cotyledons nodes of Nong cheng No.3 cucumber were pre-cultured for 3 days， inoculated with EHA-105 (D6000.3， AS 10 mg/100 mL) for 5 minutes， then co-cultured for 2 days， transformed to screening-culture medium (50 mg/L Kan， 500 mg/L Cef)， resistant buds grew well in light. 9 resistant plants were obtained and the same positive bands of 658 bp were detected by examining GUS report gene and PCR amplification of Mn-SOD gene. So the integration of the gene into cucumber genome DNA was confirmed in some degree. Key words Cucumber (Cucumis Sativus L.); Mn-SOD gene; genetic transformation
CN
EN