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IL-13 Promotes Hepatic Stellate Cell Proliferation and Collagen Synthesizing
Chen Houwen1, Wu Chao2, Li Xianglong2, Guo Mengzhou2, Xiong Zhiyong2,Fan Jie2, Zhu Mengbo3, Shi Xiaoyu4*
1The Institute of Translational Medicine, Nanchang University, Nanchang 330031, China; 2The First Clinical Medical School of Nanchang University, Nanchang 330004, China; 3The Second Clinical Medical School of Nanchang Univ
Abstract: Hepatic stellate cell is the primary source of extra ECM deposition in liver wound healing responses. Here we detected proliferation of LX-2 after exposure to IL-13 in different conditions (0~100 ng/mL×24 h or 0~48 h×10 ng/mL). Then we analyzed mRNA expression of IL-13Rα1， IL-4Rα， TGF-β and collagen type I by RT-PCR. Collagen contents in cell supernatant were also determined by hydroxyproline-assaying. In this study we report that IL-13 could promotes hepatic stellate cell proliferation with no vitiation of IL-13Rα1 and IL-4Rα gene expression. However， in experimental conditions， IL-13 were shown to unregulated TGF-β and COLA1 expression as well as collagen synthesizing which in a dose- and time-dependent manner.
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