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Local Microenvironment Determines the Primitive Streak Cell Fate in Chick Gastrulation
Wang Xiaoyu1, Li Yan1, Ma Zhenglai1, Wang Lijing2, Geng Jianguo2, Yang Xuesong1*
1Key Laboratory for Regenerative Medicine Ministry of Education, Medical College, Jinan University, Guangzhou 510632, China;2Vascular Biological Laboratory, Guangdong College of Pharmacy, Guangzhou 510224, China
Abstract: In chick gastrulation， the epiblast cells near primitive streak go through the epithelial-mesenchymal transition (EMT) and migrate into the blastocyst cavity， form the loose mesoderm at last. The cells in different part of primitive streak undergo different trajectory of cell migration. In order to understand whether the pattern of cell migration will be transformed along with the microenvironment alteration， we simply replaced the anterior or posterior primitive streak cells in host embryo using traditional transplantation technology， in which primitive streak tissue was labeled by GFP through electroporation previously in the same stage donor embryo and then we track the migration path of the GFP-positive cells with fluorescence stereomicroscope after incubation for the required time. We found that the transplanted posterior primitive streak cells to anterior part of streak followed the anterior primitive streak cell migration pattern rather than kept its posterior streak cell migration path， and so did vice versa. It suggests that the migration pattern of streak cells in gastrulation depends on the local microenvironment or spatiotemporal gene expression.
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