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Identification of Bombyx mori hsp70 Promoter and Its Function
Lan-Fang Zhuang1, Hao Wei1, Jian-Rong Lin2, Bo-Xiong Zhong1*
1College of Animal Sciences, Zhejiang University, Hangzhou 310029, China; 2College of Animal Sciences, South China of Agricultural University, Guangzhou 510642, China
Abstract: We have got two fragments located in the upstream of Bombyx mori hsp70 gene， one was hsp70- 538 (538bp in length)， and the other was hsp70-305 (305bp) through the PCR amplification. By comparing analysis， these two fragments possess of the consensus sequence of HSE (heat shock element) associated with the heat shock genes of Drosophila: CTnGAAnnTTCnAG. Applying the technology of dual-luciferase vector， these two fragments can confer heat inducement in BmN cells. And the hsp70-538 fragment can induce more activity than hsp70-305 fragment. We also informed it by transgenic analysis in silkworm. Its activity was increased with the elevated temperature. The more exposure to 42 ºC， the more amount of target gene expression we got. So these two fragments can be considered as heat shock promoters.
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