The Mechanism of MicroRNA-34a-mediated Inhibition of Human Uveal Melanoma Cell Proliferation

Abstract: MicroRNA-34a was transfected into uveal melanoma cells M23 and SP6.5 by lipofectamine. The proliferation of uveal melanoma cells was examined by BrdU and colony-forming assay， respectively， and transfection of microRNA-34a into uveal melanoma cells led to a significant decrease in cell growth (P<0.01). Flow cytometry was applied to analyze cell cycle and these cells were found to have a higher proportion of cell cycle arrest at the G1 phase. The activity of caspase-3/7 had no significant changes. In addition， the expression of microRNA- 34a in uveal melanoma cells after treatment with adriamycin was upregulated based on real-time PCR (P<0.01). The activity of caspase-3/7 increased significantly after microRNA-34a transfection and treatment with adriamycin (P<0.01). These results indicate that microRNA-34a inhibits proliferation of uveal melanoma cells by cell cycle arrest. Furthermore， microRNA-34a increases cell sensitivity to adriamycin， but doesn’t induce apoptosis directly.

CSTR: 32200.14.cjcb.2011.05.0007