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Super Interferon alpha Surpress the Proliferation of A549 Cells and Induce Cellular Senescence
Hui-Ling Li1,2, Dong-Qin Yang2, Xin Cao2, Hong-Ling Huang2, Miao Ding2, Hai-Neng Xu2, Xin-Yuan Liu2,Ming-Yang Li1*
1Department of Horticulture and Garden, the Chongqing Engineering Research Center for Floriculture, Southwestern University, Chongqing 400715, China; 2Institute of Biochemistry and Cell Biology, Shanghai Institute of Biological Scien
Abstract: In this study， the anti-proliferation effect of sIFNα was tested via the MTT assay and crystal violet staining assay. The results showed that sIFNα had a strong anticancer effect while IFNα2b had a limited effect. After treated with sIFNα， cells possessed enlarged cell size and flatted morphologic and without nucleus morphologic alteration by Hoechst 33258 staining， and there was no detection of apoptosis. At the same time， most of sIFNα-treated cells exhibited SA-β-gal positive and with the up-expression of p53 and p21. However， cells treated with IFNα2b had little or no changes and few of them were SA-β-gal positive. In conclusion， compared with IFNα2b， sIFNα exerted a potent antineoplastic effect and the mechanism might be related to its induction of cellular senescence.
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