The Effect of Three Different Factors on Extracellular Ca²⁺ Flux of Mantle Cell from Hyriopsis cumingii

Abstract: In order to study the molecular mechanisms of calcium metabolism in mantle cell from Hyriopsis cumingii， Ca²⁺ flux direction and rate were detected by non-invasive micro-test technology (NMT). Three mantle cell cultures were divided into three groups (3 reduplication/each group). For the first group， the different concentrations of Ca²⁺ (0.5 mmol/L， 1.25 mmol/L， 3 mmol/L) were added to culture medium; for the second group， the different concentrations of vitamin D3 (control， 50U/L， 100U/L， 500 U/L) were added into culture medium; for the third group， cells and tissues were cultured in the different temperatures (20ºC， 26ºC， 30ºC)， respectively. The results showed that extracellular Ca²⁺ flux was gradual changed from efflux to influx， and movement rate increased when adding Ca²⁺ into culture medium. The Ca²⁺ flux of 3 mmol/L Ca²⁺ adding group had significant difference compared with the control group (P<0.01). The movement of Ca²⁺ was gradual changed from efflux to influx， and movement rate increased when adding vitamin D3 in cultured cells. Among of this， the Ca2+ flux of 500 U/L vitamin D3 group had significant difference compared with the control group (P<0.01). Furthermore， culture temperature significantly affected the extracellular Ca2+ flux of the mantle cells. The flow directions of extracellular Ca2+changed from efflux to influx with increasing temperature (P<0.05). The influx rate at 26ºC was the biggest among all the groups (P<0.05). This experiment has a great significance on clarifing the formation and formation mechanism of pearls.

CSTR: 32200.14.cjcb.2010.05.0012