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Effects of Follicle-stimulating Hormone and Estrogen on Proliferation of Mouse Spermatogonial Cells in vitro
Da-Lei Zhang1, Ling Zhou2, Ting Zou1* , Bei Yang1, Lei Wu1
1Medical College of Nanchang University, Nanchang 330006, China; 2Nanchang Medical School, Nanchang 330006, China
Abstract: The serum-free spermatogonium-somatic cell coculture model was used to evaluate the effects of follicle-stimulating hormone (FSH) and epidermal 17β-estradiol (E2) on proliferation of mouse type A spermatogonial cells. Results showed that FSH (10 ng/ml) significantly increased spermatogonial colony number and the proliferating effect was blocked by PKA inhibitor H89. These results suggested that FSH stimulated the proliferation of mouse spermatogonia involving activation of PKA signaling pathway. E2 (10-7~10-6 mol/L) promoted proliferation of type A spermatogonia and the promoting effect of E2 was inhibited estrogen receptor antagonist tamoxifen (0.1 mg/mL). These results indicated that E2 exerted direct estrogenic action to regulate spermatagonial development via estrogen receptors expressed in the testis. Furthermore， FSH (10 ng/ml) could synergize with E2 (10-8~10-7 mol/L) to promote spermatagonial proliferation.
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