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mTERT-targeted RNA Interference in EL-4 Lymphoma Cells of Mice
Min Zhang, Xin-Xin Guo, Geng-Si Xi*
College of Life Sciences, Shaanxi Normal University, Xi’an 710062, China
Abstract: In order to investigate the expression inhibition and gene regulation of telomerase reverse transcriptase(TERT) in tumour cells of mice， designed short hairpin RNA targeted mTERT was synthesized and transfected into EL-4 lymphoma cells of mice through cationic liposomes. Further analysis of RT-PCR and Western blot indicated that the mTERT specialized shRNA effectively repressed the expressions of endogenous mTERT mRNA and protein after transfection， which made the targeted genes partially silent. MTT experiments demonstrated that the amount of EL-4 cells did not show significant difference between the sample treated with TERT specialized shRNA and the control， which may due to the phenotypic lag phenomenon caused by the inhibition of telomerase.
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