Home > Browse Issues > Vol.32 No.3
The Effects of Aging on Intracellular GSH Level and Development Ability of Ovine Oocytes
Xiao-Fang Ye1,2, Jing-Bo Chen2, Xue-Feng Lü2, Shi-Bin Chen2, Jun-Cheng Huang 2*
1College of Animal Science and Technology, Shihezi University, Shihezi 832000, China;2Xinjiang Academy of Animal Sciences, Urumqi 830000, China
Abstract: Oocytes were aging with the prolonged maturation period. Aging is detrimental to oocytes quality and subsequent development potentiality. Therefore， efficient application of the biotechnology was negatively affected by aging. This present study discussed the effects of aging on intracellular GSH level and development ability of ovine oocytes after parthenogenetic activation， SCNT and ICSI. The results has shown that: (1)ovine oocytes are aged when matured until 30hpm; (2)oocytes quality and subsequent development ability are declined by aging; (3) no significant effect on GSH level was observed by aging. In conclusion， oocyte quality can be modulated and development of embryos can be improved by controlling the maturation period or adjusting the timing of manipulation. The results provide the basis for improving treatments related delaying aging to produce more healthy embryos and preventing failures or abnormalities associated with ART using aged oocytes.
CN
EN