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Mitosis in Primary Cultures of Silkworm
Yu-Bin Ding, Min-Hui Pan, Shi-Quan Xiao, Min Chen, Xi-Jun Hong, Cheng Lu*
The Key Sericulture Laboratory of Agriculture Ministry, College of Sericulture and Biotechnology, Southwest Agriculture University, Chongqing 400716, China
Abstract: The explants used for primary culture are tissue and semi-digestive tissue， which originate from the late embryonic tissue of silkworm (Bombyx mori). The optimal conditions for primary culture are Grace's medium supplemented with moderate amount of antibiotics， 20% fetal bovine serum and incubated at temperature 28 ℃， pH 6.4-6.8. The whole process of mitosis and abnormal mitosis such as abnormal cell division plate and endonuclear mitosis were found when tissue had been cultured more than 6 months. With the time of culture prolonged， divide ratio increased from 0.02±0.01 to 0.35±0.10， while the percentage of abnormal mitosis decreased from 8.0±1.6 to 3.2±1.0.
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