Effects of miR-425-5p on Viability and Apoptosis in Glioma Cells via Targeting RAB2B
FAN Xiangyun1, ZHANG Qiang1,2*
This study was to investigate the effects of miR-425-5p on proliferation, migration, invasion and apoptosis in glioma cells via targeting RAB2B. Glioma SHG44 cells were divided into Normal control group (NC), Negative transfection group (miR-425-5p control) and miR-425-5p transfection group (miR-425-5p mimic), and transfected with DMEM culture medium, miR-425-5p control and miR-425-5p mimic, respectively. The relative expression of RAB2B and miR-425-5p after transfection in glioma SHG44 cells was determined by qRT-PCR. The proliferation, migration, invasion and apoptosis of SHG44 cells after transfection were determined. The expression of CDK2, Cyclin E1, P21, MMP-2, MMP-9 and RAB2B protein of glioma SHG44 cells after transfection was de termined by Western blot method. This study showed: (1) the level of miR-425-5p in glioma SHG44 cells decreased significantly than that in Normal human astrocytes (HA) (P<0.05), and the mRNA level of RAB2B increased signif icantly (P<0.05); (2) the level of miR-425-5p in glioma SHG44 cells transfected with miR-425-5p mimic increased significantly (P<0.05), and the mRNA level of RAB2B decreased significantly (P<0.05); (3) The proliferation of glioma SHG44 cells increased significantly at 48 h and 72 h after transfected with miR-425-5p mimic (P<0.05); (4) the healing rate of SHG44 cells transfected with miR-425-5p mimic decreased significantly (P<0.05); (5) the number of invasive cells SHG44 cells transfected with miR-425-5p mimic significantly decreased (P<0.05); (6) the apoptosis rate of glioma SHG44 cells transfected with miR-425-5p mimic increased significantly (P<0.05); (7) the level of CDK2, Cyclin E1, MMP-2 and MMP-9 in glioma SHG44 cells transfected with miR-425-5p mimic in creased significantly (P<0.05), and the levels of P21 decreased significantly (P<0.05); (8) detection of dual lucifer ase report assay showed that miR-425-5p could target the expression of RAB2B. This study proved that MiR-425 5p may inhibit the proliferation, invasion and migration of glioma cells and promote the apoptosis of glioma cells by targeting down-regulation of RAB2B.
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