PagGA2oxs Gene Family Identification and Salt Stress Response Analysis

REN Yingying¹, MAO Jianfeng^1,2, AN Xinmin¹

(¹State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, Advanced Innovation Center for Tree Breeding by Molecular Design, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland Administration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China; ²Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, Umeå 526040, Sweden)

Abstract:

GA2ox family of enzymes plays pivotal roles in plant growth and development, as well as in the response to abiotic stress. This study aims to systematically identify the members of the GA2ox family in poplar and analyze their expression patterns. Based on the previously published 84K poplar genome data, 33 GA2ox genes were identified in 84K poplar using bioinformatics methods. A systematic analysis of the gene family members was further conducted, including chromosomal localization, gene structure, physicochemical properties, cis-acting ele ments, collinearity relationships, expression patterns and protein-protein interaction networks. The results indicate that PagGA2oxs gene members can be organized into two subfamilies with similar gene structures and conserved domains. Analysis of the cis-acting elements in the promoter regions revealed that these genes are enriched in cis acting elements associated with stress and hormone responses, as well as plant growth and development. Collinear ity analysis indicated 19 collinear events between 84K poplar and Arabidopsis, suggesting clues for understanding the evolution of the GA2ox gene family. Furtherly, expression pattern analysis highlighted higher expression levels of this gene family in roots, stems and internodes. Additionally, PagGA2ox6, PagGA2ox10, PagGA2ox14, Pag GA2ox15, PagGA2ox25, PagGA2ox26, PagGA2ox32 were found to be involved in responding to salt stress and in teracting with DELLA proteins. These findings provide valuable insights into understanding the function of GA2ox genes in woody plants and deciphering salt tolerance mechanisms and also offer potential candidate genes for the development of salt-tolerant tree varieties.

CSTR: 32200.14.cjcb.2025.06.0002