Research Progress and Application of Germ Cell Line Establishment
HU Zhichao, CHEN Huifang, CAI Jianfeng, BAI Yinshan*
Cell line establishment is a technology that specific types of cells can proliferate and passage for many times in vitro, and keep the main biological characteristics of the original cells. Most studies have found that primary cells are easy to differentiate prematurely in vitro. But cell cycle operation can be activated by transfecting exogenous genes or adding specific nutritional factors, and signal pathways can be regulated, to achieve the cells proliferation and establish cell lines. Germ cell lines are mainly derived from cells in ovarian and testicular tissues. They have always been the research hotspots in cell biology, developmental biology and transgenic animals. They are also the tissues with the most cell lines. In order to lay a foundation for the research and application of cell line establishment and reproductive development, this article will review the related technology of cell line establishment
and the establishment of germ cell line related to reproduction.
CN
EN