Effects of Dextromethorax on Glioma Cell Activity under Hypoxia
CHEN Dong, ZENG Zhaoyang*
The aim of the study was to investigate the effects of dexmedetomidine on proliferation, apoptosis, migration and invasion of U87 glioma cell under hypoxic and normoxic conditions. Cobalt chloride (CoCl2, 400 μmol/L) was used to simulate hypoxia conditions. The study observed the proliferating effect of dexmedetomidine at different concentrations (0, 5, 10, 20, 40, 80 μmol/L) during different time (24, 48,72 h) on U87 glioma cells under normoxia and hypoxia by CCK-8, and flow cytometry was used to detect cell apoptosis and cell cycle changes. Migration and invasion abilities were detected by Transwell. Western blot was used to detect the changes of each proteins. Results showed that hypoxia treatment inhibited the proliferative and anti-apoptotic effects of dexmedetomidine on U87, but promoted the migration and invasion of dexmedetomidine on U87. After hypoxia treatment, the phosphorylation level of AKT was decreased. The phosphorylation level of ERK1/2 was increased, and the cyclins and apoptotic proteins were also partially changed. In addition, dexmedetomidine promoted the expression of MMP7 and MMP9 under hypoxia. In conclusion, hypoxia inhibits the pro-proliferation and anti-apoptotic effects of dexmedetomidine on glioma cells, but assists dexmedetomidine in promoting cell migration and invasion.
CN
EN