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Macrophage: An Ideal Teaching Material for Cytoskeleton Observation


XUE Yarong*, ZHUANG Zhong, HOU Dongxia, WANG Shuijuan

(School of Life Science,Nanjing University, Najing 210023, China)
Abstract:

Cytoskeleton is one of the important structure of cells, also is the important content of the course of Cell Biology Experiment. However, there is a lack of ideal cell materials for cytoskeleton observation in the current teaching practice. According to our understanding of the characteristics of easy adhesion and operation of macrophages, we used mouse abdominal macrophages as materials to observe cytoskeleton for experimental exploration. The results showed that clear cytoskeleton structure could be observed whether the cells were treated with Triton X-100 and then stained with coomassie brilliant blue or the cells were fixed, permeabilized, and stained with immunofluorescence. It shows that macrophage is an ideal teaching material for cytoskeleton observation.


CSTR: 32200.14.cjcb.2019.09.0014