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An Exploration of HRP and TMB Colorimetric Method of the Flow Cytometry MoFlo AstriosEQ on Single-Cell Sorting
Fu Rong, Zhang Xiaocui*
Core Facility of Basic Medical Sciences, College of Basic Medical Sciences of Shanghai Jiao Tong University, Shanghai 200025, China
Abstract: A single-cell sorting method was explored using flow cytometry MoFlo AstriosEQ combined with the colorimetric principle of horseradish peroxidase and 3，3′，5，5′-tetramethylbenzidine. The results showed that sorting of different calibration beads or cells resulted in a different blue change of the two chromogenic reaction， while the unsorted control groups were colorless， which were significantly different between the groups in D650 value. However， sorting calibration beads or cells have not significant difference. It concluded that the method could quickly judge whether the actual number of beads or cells sorted into microtiter plate wells in accordance with the theoretical setting， and provide accurate information for the single cell sorting. In addition， the whole verification process did not require large-scale or special instruments and the reagent was cheap， which could be used as an auxiliary means for single cell sorting experiments in the future.
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