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Role of ROS in Cadmium-Induced Oxidative Damage and Apoptosis in HK-2 Cells
Dong Feng, Wu Qiong, Li Xiangyang, Zhang Shaoying, Wang Lan*
College of Life Science, Shanxi University, Taiyuan 030006, China
Abstract: In this study， the role of ROS in Cadmium-induced oxidative damage and apoptosis in HK-2 cells was studied. The oxidative damage of CdCl2 on HK-2 cells was detected and evaluated by MTT assay， DCFH-DA labelling， JC-1 staining， comet assay and flow cytometry analysis. The results showed that CdCl2 treatment significantly suppressed viability of HK-2 cells and induced morphological changes in a time- and concentration-dependent manner. CdCl2 treatment increased ROS levels， reduced mitochondrial membrane potential Δψm， induced DNA damage and triggered caspase-3 activation， resulting in HK-2 cell apoptosis. In addition， combination of CdCl2 and ROS scavenger NAC， obviously improved the cell morphology， significantly reduced ROS levels， increased the mitochondrial membrane potential Δψm， decreased DNA damage and apoptosis (P<0.01). Taken together， these data suggested that ROS mediated Cadmium-induced oxidative damage and apoptosis in HK-2 cells.
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