The Application of Glutaraldehyde and Paraform Mixed Fixative for Specimens Used for Immunohistochemistry and Transmission Electron Microscope Observation

Tao Yaqun, Li Guiping, Yi Jing, Yang Jie^*

Department of Biochemistry and Molecular Cell Biology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China

Abstract: The present study introduces a technical experience to figure out a suitable ratio of glutaraldehyde and paraform in the perfusing fixative for specimens used for both immunohistochemistry and transmission electron microscope (TEM) observation in the same experimental animal. The heart of mouse was perfused with different ratios of fixative respectively. The myocardium， kidney and liver were then observed by TEM for ultrastructural changes. In addition， the myocardial tissue was selected for immunohistochemical reaction and immune colloidal gold labeling. The results showed that 0.10% glutaraldehyde+4% paraform mixture is suitable for preservation of the ultrastructure and the antigen activities.

CSTR: 32200.14.cjcb.2016.12.0011