The Study in Mechanism of Rapamycin on Suppressing Inflammation via Akt/NF-κB Signaling Pathway in Renal Tissue of Diabetic Rats

Abstract: This article explored the mechanisms of rapamycin on suppressing inflammation in renal tissue of diabetic rats. 30 male SD rats were randomly divided into 3 groups: control group， diabetes group and diabetes with rapamycin treatment group (1 mg/kg/d). Streptozotocin (65 mg/kg) was injected to induce diabetes in rats. After 2 days of streptozotocin injection， rats in diabetes with rapamycin treatment group received subcutaneous injection of rapamycin， while rats in control and diabetes groups were injected an equal volume of saline. After 6 weeks，it was found that the levels of creatinine and urea nitrogen in serum increased in diabetic rats (P<0.05)， which could reduced by rapamycin treatment. Hematoxylin and eosin staining showed that glomerular capsule was narrowed，the integrity of basement membrane was disturbed， tubular epithelial cell was hypertrophic in diabetic rats.Meanwhile， the expression levels of tumor necrosis factor-α (TNF-α)， interleukin-1β (IL-1β)， interleukin-6 (IL-6)， phosphorylated protein kinase B (p-Akt) and nuclear transcription factor-κB p65 (NF-κB p65) were significantly increased in renal tissue of diabetic rats. However， the pathological structure was alleviated and the levels of TNF-α，IL-1β， IL-6， p-Akt， NF-κB p65 were significantly reduced by rapamycin treatment. These results suggested that rapamycin could ameliorate renal dysfunction in diabetic rats， which might be related to suppress inflammation via Akt/NF-κB signaling pathway in renal tissue.

CSTR: 32200.14.cjcb.2015.09.0010