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Regulation of Mouse Monocyte/Macrophages Phagocytosis by Sphingosine 1-Phosphate
Zhang Yuanyuan, Li Weiyang, Liu Xin, Li Liying*
College of Basic Medicine, Capital Medical University, Beijing 100069, China
Abstract: Macrophage phagocytosis is an essential step in innate immunity. However， the signals regulating the phagocytosis are still not fully understood. This study aimed to evaluate the effects of S1P and S1P receptors (S1PRs) on macrophage phagocytosis in vitro. We found that S1PR1， S1PR2 and S1PR3 were all expressed in monocyte/ macrophages RAW264.7. A powerful phagocytosis activity was exerted by S1P on monocyte/macrophages and the effects of pro-phagocytosis were markedly inhibited by S1PR2 or S1PR3 antagonists or siRNAs， but not S1PR1 antagonist or siRNA. Interestingly， exogenously added S1P induced significant up-regulation of S1PR2/3， but not affected S1PR1， suggesting a self-amplifying loop of S1P to enhance macrophage phagocytosis activity. In conclusion，the results revealed that S1P/S1PR2/3 signaling axis stimulated phagocytosis activity in mouse monocyte/macrophages and provided new clues for the molecular mechanisms of macrophage phagocytosis.
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