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Detection and Analysis of Proteomic Patterns of Human Adenocarcinoma Cell Lung Cancer A549 Treated with Radiation
Xiang-Lan Li1, Jin Chang1, Hong-Tao Hu1, Qing-Shan You*, Yu-Yan Ma2
1Department of Radiation Oncology, 2Tumor Institute, Tumor Hospital, Harbin Medical University, Harbin 150081, China
Abstract: To screen differential expression protein of human lung adenocarcinoma cell A549 treated with radiation and to discuss the role of these proteins. Differential expression protein in A549 cell treated with radiation were screened by 2D-PAGE and identified by MALDI-TOF-MS. Twenty-five differential expression protein spots were found in A549 cell treated with radiation by 2D-PAGE， and the six proteins (manganese superoxide dismutase isoform B precursor， keratin 9， dystrobrevin， HNRPH1， enolase 1， keratin 8) were identified by MALDI-TOF-MS. The differential expression protein between the treated with radiation by 2D-PAGE relate to antioxidation， radioprotection， signal transmission， RNA processing and transfering， cell apoptosis. It is possibly significance for explaining molecule mechanism of the responds of cell to radiation.
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