The TBP-like Protein (TLP) Inhibits the Proliferation of HeLa Cells through G2/M Phase Arrest

Abstract: TBP-like protein (TLP)， one kind of transcription factors in eukaryotic cells， plays an important role in regulating growth and development. In this experiment， we constructed the recombinant plasmids of pEGFP-N1-TLP to study the effect of TLP gene on the proliferation of HeLa cells. The transfection efficiency of plasmids was detected with flow cytometry and the subcellular localization of exogenous TLP protein was determined through laser scanning confocal microscope. TLP gene function of proliferation inhibition was determined by MTT assay， gene silence induced by RNAi-TLP and Hoechst33258 staining. Flow cytometry， Western blot and RT-PCR assay showed that TLP induced cell cycle arrest of HeLa cells in G2/M phase， by inhibiting the transcription and expression levels of CDK1 and CyclinB1 genes. In conclusion， this study showed that the exogenous TLP， expressing exclusively in nucleus of HeLa cells， inhibited the proliferation of HeLa cells through G2/M phase arrest.

CSTR: 32200.14.cjcb.2014.04.0004